diff --git a/setuptools/dist.py b/setuptools/dist.py
index 886e1e6c831..118198d0b07 100644
--- a/setuptools/dist.py
+++ b/setuptools/dist.py
@@ -413,7 +413,10 @@ def _finalize_license_files(self) -> None:
             patterns = ['LICEN[CS]E*', 'COPYING*', 'NOTICE*', 'AUTHORS*']
 
         self.metadata.license_files = list(
-            unique_everseen(self._expand_patterns(patterns))
+            map(
+                lambda path: path.replace("\\", "/"),
+                unique_everseen(self._expand_patterns(patterns)),
+            )
         )
 
     @staticmethod
diff --git a/setuptools/tests/test_bdist_wheel.py b/setuptools/tests/test_bdist_wheel.py
index b88b2e51fc8..0f2e6ce136d 100644
--- a/setuptools/tests/test_bdist_wheel.py
+++ b/setuptools/tests/test_bdist_wheel.py
@@ -358,6 +358,9 @@ def test_licenses_override(dummy_dist, monkeypatch, tmp_path, config_file, confi
             for fname in {"licenses/DUMMYFILE", "LICENSE"}
         }
         assert set(wf.namelist()) == DEFAULT_FILES | license_files
+        metadata = wf.read("dummy_dist-1.0.dist-info/METADATA").decode("utf8")
+        assert "License-File: licenses/DUMMYFILE" in metadata
+        assert "License-File: LICENSE" in metadata
 
 
 def test_licenses_preserve_folder_structure(licenses_dist, monkeypatch, tmp_path):
@@ -371,6 +374,9 @@ def test_licenses_preserve_folder_structure(licenses_dist, monkeypatch, tmp_path
             "licenses_dist-1.0.dist-info/licenses/src/vendor/LICENSE",
         }
         assert set(wf.namelist()) == default_files | license_files
+        metadata = wf.read("licenses_dist-1.0.dist-info/METADATA").decode("utf8")
+        assert "License-File: src/vendor/LICENSE" in metadata
+        assert "License-File: LICENSE" in metadata
 
 
 def test_licenses_disabled(dummy_dist, monkeypatch, tmp_path):