From 083010b06b446d1087d74861ac2080556078f450 Mon Sep 17 00:00:00 2001 From: Rhyann Clarke <146747548+rclarke0@users.noreply.github.com> Date: Fri, 10 Jan 2025 15:17:55 -0500 Subject: [PATCH] fix (abr-testing): Change reservoir size in ABR6 Protocol (#17247) # Overview Change reservoir and liquid clean up step for ABR6 protocol. ## Test Plan and Hands on Testing tested on robot x3 times. ## Changelog ## Review requests ## Risk assessment --- .../6_Omega_HDQ_DNA_Cells-Flex_96_channel.py | 7 +++---- 1 file changed, 3 insertions(+), 4 deletions(-) diff --git a/abr-testing/abr_testing/protocols/active_protocols/6_Omega_HDQ_DNA_Cells-Flex_96_channel.py b/abr-testing/abr_testing/protocols/active_protocols/6_Omega_HDQ_DNA_Cells-Flex_96_channel.py index 894f80dcdea..a3fd6251a43 100644 --- a/abr-testing/abr_testing/protocols/active_protocols/6_Omega_HDQ_DNA_Cells-Flex_96_channel.py +++ b/abr-testing/abr_testing/protocols/active_protocols/6_Omega_HDQ_DNA_Cells-Flex_96_channel.py @@ -33,7 +33,7 @@ def add_parameters(parameters: ParameterContext) -> None: parameters.add_int( variable_name="number_of_runs", display_name="Number of Runs", - default=2, + default=4, minimum=1, maximum=4, ) @@ -53,7 +53,7 @@ def run(protocol: ProtocolContext) -> None: bind_vol = 300.0 sample_vol = 180.0 elution_vol = 100.0 - helpers.comment_protocol_version(protocol, "01") + helpers.comment_protocol_version(protocol, "02") # Same for all HDQ Extractions deepwell_type = "nest_96_wellplate_2ml_deep" if not dry_run: @@ -87,7 +87,7 @@ def run(protocol: ProtocolContext) -> None: magblock: MagneticBlockContext = protocol.load_module( "magneticBlockV1", "C1" ) # type: ignore[assignment] - liquid_waste = protocol.load_labware("nest_1_reservoir_195ml", "B3", "Liquid Waste") + liquid_waste = protocol.load_labware("nest_1_reservoir_290ml", "B3", "Liquid Waste") waste = liquid_waste.wells()[0].top() lysis_reservoir = protocol.load_labware(deepwell_type, "D2", "Lysis reservoir") @@ -417,7 +417,6 @@ def clean() -> None: elutionplate, wash2_reservoir, wash1_reservoir, - liquid_waste, ] helpers.clean_up_plates(pip, plates_to_clean, liquid_waste["A1"], 1000)